Submitted

β-adrenergic signaling modulates cancer cell mechanotype through a RhoA-ROCK-myosin II axis. Kim TH, Vazquez-Hidalgo E, Chavez B, Abdou A, Tan XHM, Christodoulides A, Farris CM, Chiou PY, Sloan EK, Katira P, Rowat AC. 

Cancer growth and invasion are increased in the tight skin (TSK) mouse. Taylor-Harding B, Recouvreux MS, Rowat AC, Karlan BY, Orsulic S. 

2024

Linker Histone H1.0 Couples Cellular Mechanical Behaviors to Chromatin Structure (2024) Hu S, Chapksi DJ, Gehred N, Kimball TH, Gromova T, Flores F, Rowat AC, Chen J, Sevag Packard RR, Olszewski E, Davis J, Rau CD, McKinsey TA, Rosa Garrido M, Vondriska TM. Nature Cardiovascular Research. In press 

Scalable Processes for Culturing Meat Using Edible Scaffolds (2024) Kawecki NS, Chen KK, Smith CS, Xie Q, Cohen JM, Rowat AC. Annu Rev Food Sci Tech. In press. doi: 10.1146/annurev-food-072023-034451

2023

Engineering multicomponent tissue by spontaneous adhesion of myogenic and adipogenic microtissues cultured with customized scaffolds (2023) Kawecki NS, Norris SCP, Xu Y, Wu Y, Davis AR, Fridman E, Chen KK, Crosbie RH, Garmyn A, Li S, Mason T, Rowat AC.. Food Research International. 172: 113080 

Altered physical phenotypes of leukemia cells that survive chemotherapy treatment (2023) Ly C, Ogana H, Kim HN, Hurwitz S, Deeds EJ, Kim YM, Rowat AC. Integrative Biology. 15: zyad006. 

Reduction of Intracellular Tension and Cell Adhesion Promotes Open Chromatin Structure and Enhances Cell Reprogramming (2023) Soto J, Song Y, Chen B, Park H, Akhtar N, Hoffman T, Ly C, Sia J, Wong SY, Kelkhoff DO, Chu J, Poo MM, Downing TL, Rowat AC, Li S. Advanced Science. e2300152 

Kawecki NS, Rowat AC (2023) Future Pathways in Biosynthesis: Livestock Tissue Culture in ‘The Protein Transition’. Eds. Pyett S, van Mierlo B, Trindade L, de Vet E, Welch D, van Zanten H, de Jong L, and van den Top M [Invited Book Chapter]  

2022

Multi-facet roles of RNA editing in promoting loss-of-function of PODXL in cancer. Fu T, Chan TW, Bahn JH, Kim TH, Rowat AC, Xiao X (2022) iScience. 25: 104836.

Emulsion-templated microparticles with tunable stiffness and topology: applications as edible microcarriers for cultured meat. Norris SCP, Kawecki NS, Davis AR, Chen KK, Rowat AC (2022) Biomaterials. 287: 121699.

Transient nuclear deformation preconditions epigenetic state and promotes cell reprogramming. Song Y, Soto J, Chen B, Hoffman T, Zhao W, Zhu N, Peng Q, Liu L, Ly C, Wong PK, Wang Y, Rowat AC, Kurdistani SK, Li S (2022) Nature Materials. 21: 1191-1199.

Chemo-mechanical factors that limit cellular force generation. Vazquez-Hildalgo E, Farris C, Rowat AC, Katira P (2022) Frontiers in Physics [https://doi.org/10.3389/fphy.2022.831776]

Fluidic methods to measure cellular deformability. Ly C, Kim TH, Rowat AC (2022) In ‘Mechanics of Diseases: Biomedical Aspects of the Mechanical Properties of Cells and Tissues’. Eds. Radmacher M, Lekka M, Podestà A, Navajas D. [Invited Book Chapter]

Perspectives on Scaling Production of Adipose Tissue for Food Applications. Yuen J, Theodossiou S, Stout A, Kawecki NS, Letcher S, Cohen J, Barrick B, Saad M, Rubio N, Pietropinto J, DiCindio H, Rowat AC, Kaplan D (2022) Biomaterials. 280: 121273. [Review]

Cancer Cell Mechanobiology: A New Frontier for Cancer Research. Yu W, Sharma S, Rao E, Rowat AC, Gimzewski JK, Han D, Rao J (2022) Journal of the National Cancer Center. 2: 10-17. [Review]

2021

Nuclear membrane ruptures underlie the vascular pathology in Hutchinson-Gilford progeria syndrome. Kim PH, Chen NY, Heizer PJ, Tu Y, Weston TA, Gill NK, Rowat AC, Young SG, Fong LG (2021) JCI Insight. 6: e151515.

2020

Type V collagen in scar tissue regulates the size of scar after heart injury. Yokota T, McCourt J, Ma F, Ren S, Li S, Kim TH, Kurmangaliyev YZ, Ahadian S, Nasiri R, Nguyen T, Haw M, Zhou Y, Wu R, Rodriguez A, Cohn W, Wang Y, Whitelegge J, Ryazantsev S, Khadamhosseini A, Teitell MA, Chiou E, Birk D, Rowat AC, Crosbie R, Pellegrini M, Seldin M, Lusis AJ, Deb A (2020) Cell. 182: 545-562.e23. (pdf)

RhoA-myosin II axis protects circulating tumor cells from fluid shear stress-induced damage. Moose DL, Krog BL, Zhao L, Kim TH, Burke G, Rhodes L, Vanneste V, Breheny P, Milhem M, Rowat AC, Henry MD (2020) Cell Reports. 30: 3864–3874. (pdf)

Differential contributions of actin and myosin to the physical phenotypes and invasion of pancreatic cancer cells. Nguyen AV, Trompetto B, Tan XHM, Scott MB, Hu KH, Deeds E, Butte MJ, Chiou PY, Rowat AC (2020) Cellular and Molecular Bioengineering. 13: 27-44. (pdf)

Bridging the gap between the science of cultured meat and public perceptions. Tomiyama AJ, Kawecki NS, Rosenfeld D, Jay JA, Rajagopal D, Rowat AC (2020) Trends Food Sci Tech.104: 144-152. [Review] (pdf)

Unraveling the mechanobiology of immune cells. Zhang X, Kim TH, Ly C, Li H, Butte M, Gu Z, Rowat AC, Li S (2020) Current Opinion in Biotechnology, 66: 236-245. [Review] (pdf)

Single Cell Mechanotype and Associated Molecular Changes in Urothelial Cell Transformation and Progression (2020) Yu,W, Lu QY, Sharma S, Ly C, Di Carlo D, Rowat AC, LeClaire M, Kim D, Chow C, Gimzewski JK, Rao J. Frontiers Cell Develop Biol., 8: 601376. (pdf)

Challenges, Opportunities, and Motivators for Developing and Applying Food Literacy in a University Setting, A Qualitative Study. Malan H, Watson TD, Slusser W, Glik D, Rowat AC, Prelip M (2020) Journal of the Academy of Nutrition and Dietetics. pii: S2212-2672(19)30515-5. (pdf)

2019

Promoting an interdisciplinary food literacy framework to cultivate critical citizenship. Rowat AC, Soh M, Schmidt L, Jensen L, Malan H, Slusser W (2019) J Am College Health. 5: 1-4. (pdf)

A Study of High-Grade Serous Ovarian Cancer Origins Implicates the SOX18 Transcription Factor in Tumor Development. Lawrenson K, Fonseca MAS, Liu AY, Segato F, Lee JM, Lin X, Corona RI, Abbasi F, Kevin C. Vavra KC, Dinh HQ, Gill NK, Seo JH, Coetzee S, Lin YG, Pejovic T, Mhawech-Fauceglia P, Rowat AC, Drapkin R, Karlan BY, Hazelett DJ, Freedman ML, Gayther SA, Noushmehr H (2019) Cell Reports. 29: 3726-3735. (pdf)

Microfluidic Mechanotyping of a Single Cell with Two Consecutive Constrictions of Different Sizes and an Electrical Detection System. Sano M, Kaji N, Rowat AC, Yasaki H, Shao L, Odaka H; Yasui T, Higashiyama T, Baba Y (2019) Analytical Chemistry. 91: 12890-12899. (pdf)

DYT1 dystonia patient-derived fibroblasts have increased deformability and susceptibility to damage by mechanical forces. Gill NK, Ly C, Kim P, Fong LG, Young SG, Saunders CA, Luxton GWG, Rowat AC (2019) Frontiers Cell Develop Biol. 7: 103. (pdf)

Stress hormone signaling through β-adrenergic receptors regulates macrophage mechanotype and function. Kim TH, Ly C, Christodoulides A, Nowell CJ, Gunning PW, Sloan EK, Rowat AC (2019) FASEB J. 33: 3997–4006. (pdf)

Activating p53-family member TAp63: A novel therapeutic strategy for targeting p53-altered tumors. Gunaratne PH, Pan Y, Rao AK, Lin C, Hernandez-Herrera A, Liang K, Rait AS, Venkatanarayan A, Benham AL, Rubab F, Kim SS, Rajapakshe K, Chan CK, Selanere M, Sood A, Rowat AC, Coarfa C, Pirollo KF, Flores ER, Chang EH (2019) Cancer. 125: 2409-2422. (pdf)

A scalable filtration method for high throughput screening based on cell deformability. Gill NK, Ly C, Nyberg KD, Lee L, Qi D, Tofig B, Sobreiro MR, Dorigo O, Rao JY, Wiedemeyer R, Karlan B, Lawrenson K, Freeman MR, Damoiseaux R, Rowat AC (2019) Lab Chip. 19: 343 - 357. (pdf)

2018

Emerin deregulation links nuclear shape instability to metastatic potential. Sobreiro MR, Chen JF, Novitskya T, You S, Morley S, Steadman K, Gill NK, Eskaros A, Rotinen M, Chu CY, Chung LWK, Tanaka H, Yang W, Knudsen BS, Tseng HR, Rowat AC, Posadas EM, Zijlstra A, Di Vizio D, Freeman MR (2018) Cancer Research. 78: 6086-6097. (pdf)

Predicting cancer cell invasion by single-cell physical phenotyping. Nyberg KD, Bruce SL, Nguyen AV, Chan CK, Gill NK, Kim TH, Sloan EK, Rowat AC (2018) Integrative Biol. 10: 218-231. (pdf)

2017

Quantitative deformability cytometry (q-DC): rapid measurements of single cell viscoelastic properties (2017) Nyberg KD, Hu KH, Kleinman SH, Khismatullin DB, Butte MJ, Rowat AC (2017) Biophysical Journal. 113: 1574-1584. (pdf)

Wrinkling of milk skin is mediated by evaporation. Evans AA, Cheung E, Nyberg KD, Rowat AC (2017) Soft Matter. 13: 1056-1062. (pdf)

A protocol for screening cells based on deformability using parallel microfiltration. Gill NK, Qi D, Kim TH, Chan CK, Nguyen AV, Nyberg KD, Rowat AC (2017) Nature Protocol Exchange. (link)

2016

Cancer cells are less deformable and more invasive with activation of β-adrenergic signaling. Kim TH, Gill NK, Nyberg KD, Nguyen AV, Hohlbauch SV, Geisse NA, Nowell CJ, Sloan E, Rowat AC (2016) J Cell Sci. 129: 4563-4575. (pdf)

Tumour-suppressor microRNAs regulate ovarian cancer cell physical properties and invasive behaviour. Chan CK, Pan Y, Nyberg KD, Lim E, Robertson G, Maar D, Gunaratne PH, Rowat AC (2016) Royal Society Open Biology. 6: 160275. (pdf)

Stiffness of pancreatic cancer cells is associated with increased invasive potential. Nguyen AV, Nyberg KD, Scott MB, Nguyen A, Hohlbauch SV, Geisse NA, Robertson G, Gibb E, Welsh A, Wu N, Donahue T, Rowat AC (2016) Integrative Biology. 8: 1232 – 1245. (pdf)

The physical origins of transit time measurements for rapid, single cell mechanotyping. Nyberg KD, Scott MB, Bruce SL, Gopinath AB, Bikos D, Mason TG, Kim JW, Choi HS, Rowat AC (2016) Lab Chip. 16: 3330 - 3339. (pdf)

Neural regulation of cancer: from mechanobiology to inflammation. Kim TH, Rowat AC*, Sloan E* (2016) Clinical and Translational Immunology. 5: e78. (pdf)

miR-509-3p is clinically significant and strongly attenuates cellular migration and multi-cellular spheroids in ovarian cancer. Robertson AG, Pan Y, Pedersen L, Lim E, Hernandez-Herrera A, Rowat AC, Chan CK, Chu A, Wen Y, Zhang X, Patil SL, Basu-Roy U, Mansukhani A, Sipahimalani P, Bowlby R, Brooks D, Thiessen N, Ma Y, Moore RA, Schein JE, Mungall AJ, Pecot CV, Sood AK, Jones SJM, Marra MA, Gunaratne P (2016) Oncotarget. 7: 25930-25948. (pdf)

2015

Screening cell mechanotype using parallel microfiltration. Qi D^•, Gill NK^•, Santiskulvong C, Dorigo O, Rao J, Taylor-Harding B, Wiedemeyer W, Rowat AC (2015) Sci. Reports. 5: 17595. (pdf)

Physical cell properties that influence migration in confined 3D environments. Lautscham LA, Kämmerer C, Lange J, Kolb T, Mark C, Schilling A, Strissel P, Strick R, Gluth C, Rowat AC, Metzner C, Fabry B (2015) Biophys. J. 109: 1-14. (pdf)

Understanding Fick’s law and diffusion theory through food & cooking. Zhou L, Nyberg K, Rowat AC (2015) Adv. Physiol. Edu. 39: 192–197. (pdf) [Editor’s Pick; Featured in American Physiological Society’s ‘I Spy Physiology’ blog]

A large displacement, high frequency, underwater MEMS actuator. Hoelzle DJ, Chan CK, Scott M, Rowat AC (2015) J. Applied Phys. 117: 014503. (pdf)

Interactive taste tests enhance student learning. Soh M, Roth-Johnson EA, Levis M, Rowat AC (2015) J. Coll. Sci. Teaching. 45: 44-48. (pdf)

2014

The kitchen as a physics classroom. Rowat AC, Sinha NN, Sörensen P, Campàs O, Castells P, Rosenberg D, Brenner MP, Weitz DA (2014) Physics Education, 49: 512-522. (pdf)

A microfluidic technique to probe cell deformability, Hoelzle DJ, Chan CK, Varghese BA, Rowat AC (2014) J. Vis. Expt. 91: e51474. (link)

A new function for Coenzyme Q: A membrane stabilizer? Clarke CF, Rowat AC, Gober JW (2014) Nat. Chem. Biol. 10: 242-243. [News & Views] (pdf)

2013

Elasticity determines shape transitions in soft spheres. Fogle C, Rowat AC, Levine AJ, Rudnick J (2013) Phys. Rev. E., 8: 052404. (pdf)

Nuclear envelope composition determines the ability of neutrophil-type cells to passage through micron-scale constrictions. Rowat AC*, Jaalouk DE, Zwerger M, Ung L, Eydelnant I, Olins D, Olins A, Herrmann H, Weitz DA, Lammerding J (2013) J. Biol. Chem., 288: 8610-8. (pdf)

The molecules we eat: Food as a medium to communicate science, Rowat AC (2013) Flavour, 2: 10. (pdf)

2012

Parallel fabrication of an array of holes in PDMS. Qi D, Chan CK, Rowat AC* (2012) Lab on a Chip - Chips & Tips. (link)

Probing single cells using flow in microfluidics, Qi D^•, Hoelzle DJ^•, Rowat AC* (2012) Eur. Phys. J., 204: 85-101. (pdf)

A microfluidic approach to encapsulate living cells in uniform alginate hydrogel microparticles, Martinez CJ, Kim JW, Ye C, Ortiz I, Rowat AC, Marquez M, Weitz DA (2012) Macromolecular Bioscience, 12: 946-951. (pdf)

2011

The science of chocolate: phase transitions, emulsification, and nucleation, Rowat AC*, Hollar K, Rosenberg D, Stone HA (2011) J. Chem. Edu., 88: 29-33. (pdf)

2010

Divalent cations crosslink vimentin intermediate filament tail domains to regulate network mechanics. Lin YC, Broedersz CP, Rowat AC, Wedig T, Herrmann H, MacKintosh FC, Weitz DA (2010) J. Mol. Biol., 399: 637-44. (pdf)

Ultra high-throughput screening in drop-based microfluidics for directed evolution of highly active peroxidases. Agresti JJ, Antipov E, Abate AR, Ahn K, Rowat AC, Baret JC, Marquez MM, Klibanov AM, Griffiths AD, Weitz DA (2010) Proc. Natl. Acad. Sci., 107: 4004-9. [Featured in ACS Chem. Biol. Spotlight (2010) 5: 261–4; Faculty of 1000 Evaluation; Science Daily] (pdf)

The science of pizza: the molecular origins of cheese, bread, and digestion using interactive activities, Rowat AC*,Hollar K, Rosenberg D, Stone HA (2010) J. Food Sci. Edu., 9:106-112. (pdf)

2009

Understanding epigenetic mechanisms: tracking protein expression across multiple generations of cells. Rowat AC*, Weitz DA (2010) Eur. J. Phys., 178: 71-80. (pdf)

Tracking lineages of cells in lines using a microfluidic device. Rowat AC*, Bird J, Agresti JJ, Rando OJ, Weitz DA* (2009) Proc. Natl. Acad. Sci., 106: 18149-54. (pdf)

Electrodes on a budget: micropatterned electrode fabrication by wet chemical deposition. Ebina W, Rowat AC*, Weitz DA (2009) Biomicrofluidics. 3: 034104. [selected for publication in the Virtual Journal of Biological Physics Research (2009) 18:6] (pdf)

Fabrication of reversibly adherent fluidic devices by magnet adhesion. Rafat M, Raad D, Rowat AC, Auguste DT (2009) Lab on a Chip. 9: 3016-9. (pdf)

Dropspots: a picoliter array in a microfluidic device. Schmitz C^•, Rowat AC^•*, Köster S, Weitz DA* (2009) Lab on a Chip, 9: 44-49. (pdf)

Physical properties of the cell nucleus studied by micropipette aspiration. Rowat AC* (2009) in The Nucleus: Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging. Ed. Hancock, R. Totowa, USA: Humana Press. 464: 3-12. (pdf)

2008

Biocompatible surfactants for water-in-fluorocarbon emulsions. Holtze C, Rowat AC, Agresti JJ, Hutchison B, Angilè FE, Köster S, Duan H, Schmitz C, Humphry KJ, Pisignano D, Weitz DA (2008) Lab on a Chip. 8: 1632-9. [highlighted in Chemical Technology] (pdf)

Modular drop-based microfluidic devices for single cell studies. Köster S, Angilè FE, Duan H, Agresti JJ, Wintner A, Schmitz C, Rowat AC, Merten CA, Pisignano D, Griffiths AD, Weitz DA (2008) Lab on a Chip. 8: 1110 – 5. (pdf)

Influence of ethanol on lipid membranes: from lateral pressure profiles to dynamics and partitioning. Terama E, Ollila OHS, Salonen E, Rowat AC, Trandum C, Westh P, Patra M, Karttunen M, Vattulainen I (2008) J. Phys. Chem. B. 112: 4131-4139.

How to easily punch holes in a PDMS microfluidic device. Rowat AC*, Weitz DA (2008) Lab on a Chip - Chips & Tips. (link)

Towards an integrated understanding of the structure and mechanics of the cell nucleus. Rowat AC*, Lammerding J, Herrmann H, Aebi U (2008) BioEssays. 30: 226-36. (pdf)

Controlled emulsions: fabrication and applications. Shah RK, Shum HC, Rowat AC, Lee D, Agresti JJ, Utada AS, Chu LY, Kim JW, Fernandez-Nieves A, Martinez CJ, Weitz DA (2008) Materials Today. 11: 18-27. (pdf)

2007

The cell as a material. Kasza KE, Rowat AC, Liu J, Angelini TE, Brangwynne CP, Koenderink GH, Weitz DA (2007) Curr. Opin. Cell Biol. 19:101-7. (pdf)

2006

Mechanics of the cell nucleus and effects of emerin deficiency. Rowat AC*, Lammerding J, Ipsen JH (2006) Biophys. J. 91:4649-64. (pdf)

Universal behaviour of membranes with sterols. Henriksen JH, Rowat AC, Brief E, Hsueh YW, Thewalt JL, Zuckermann MJ, Ipsen JH (2006) Biophys. J. 90: 1639-49. (pdf)

Interactions of oleic acid and model stratum corneum membranes as seen by 2H-NMR. Rowat AC, Kitson N, Thewalt JL (2006) J. Int. Pharm. 307:225-31. (pdf)

< 2005

Effects of farnesol on the physical properties of DMPC membranes. Rowat AC, Keller D, Ipsen JH (2005) Biochim. Biophys. Acta. 1713: 29-39. (pdf)

Characterization of the elastic properties of the nuclear envelope. Rowat AC, Foster LJ, Nielsen MM, Weiss M, Ipsen JH (2005) J. Roy. Soc. Interface. 2: 63-9. [highlighted in J. Exp. Biol. (2005) 208:vii] (pdf)

Farnesylated peptides in model membranes: a biophysical investigation. Rowat AC, Brask J, Sparrmann T, Jensen KJ, Lindblom G, Ipsen JH (2004) Eur. Biophys. J. 33: 300-9. [Erratum in Eur Biophys J. 2004 Oct;33(6):562-3.] (pdf)

Farnesol-DMPC phase behaviour: a 2H-NMR study. Rowat AC, Davis JH (2004) Biochim. Biophys. Acta 661: 178-87. (pdf)

Experimental evidence of the electrostatic contribution to membrane bending rigidity. Rowat AC, Hansen PL, Ipsen JH (2004) Europhys. Lett. 67: 144-9. (pdf)

Vesicle fluctuation analysis of the effects of sterols on membrane bending rigidity. Henriksen JH, Rowat AC, Ipsen JH (2004) Eur. Biophys. J. 33: 732-41. (pdf)

Brillouin light scattering studies of the mechanical properties of polystyrene/polyisoprene multilayered thin films. Forrest J, Rowat AC, Dalnoki-Veress K, Stevens JR, Dutcher JR (1996) Journal of Polymer Science: Part B: Polymer Physics. 34: 3009-16.

Brillouin light scattering determination of the glass transition in thin, freely-standing polystyrene films. Forrest J, Dalnoki-Veress K, Dutcher JR, Rowat AC, Stevens JR (1996) Mater. Res. Soc. Symp. 407: 131-6.